This is a C# source code that generates a signed, encrypted AS2 message with a request for an MDN.  The complete program can be downloaded at EDIINT AS2
A detailed explanation of the algorithm can be read at "Creating an AS2 client in stages".

private void btnEncryptSignedMdn_Click(object sender, EventArgs e)
            ediDocument oEdiDoc = null;
            mailDocument oMailDoc = null;
            mailMessage oMailSubject = null;
            bool bSyncronous = true;

            string sPath = AppDomain.CurrentDomain.BaseDirectory;

            oEdiDoc = new ediDocument();

            //object for AS2 file
            oMailDoc = oEdiDoc.GetMailDocument();

            //Setting so that syntax for the field "Disposition-Notification-To" is not validated, but treated as a free form text field
            oMailDoc.AddDefinedHeader("Disposition-Notification-To", MailMessageHeaderTypeConstants.HeaderType_FieldText, RequirementTypeConstants.Requirement_Optional);

            //oject for subject/content in AS2 file
            oMailSubject = oMailDoc.GetMessageContent();

            //add appropriate header to describe the content
            //  - "Application/EDI-X12" for EDI X12
            //  - "Application/EDIFACT" for EDI UN/EDIFACT
            //  - "Application/XML" for XML
            oMailSubject.set_HeaderFieldValue("Content-Type", "Application/EDI-X12");

            //the content to be sent in this AS2 message is an EDI X12 file.  The file is imported into the message object.
            oMailSubject.Import(sPath + "ediFile.X12");

            // Configure the security of this message.
            ediSecurity oSecurity = oMailSubject.GetSecurity();

            // Specify the type of encryption and signing algorithm to use
            oSecurity.EncryptionAlgorithm = "3DES";
            oSecurity.AssuranceAlgorithm = "SHA-1";

            // Your trading partner's public key certificate is required to encrypt the message.
            // Recipient's certificate to encrypt message.
            oSecurity.SetCertSubjectNameByLocation("TradingPartner_Cert", "CurrentUser", "My", "Microsoft Strong Cryptographic Provider");
            // Your private key certificate is required to sign (and decrypt) the message
            // Sender's certificate to sign message.
            oSecurity.SetCertSignerNameByLocation("MyCert", "CurrentUser", "My", "Microsoft Strong Cryptographic Provider");
            oSecurity.EnableEncryption = true;

            //if signed, enable assurance and calculate MIC
            oSecurity.EnableAssurance = true;

            txtMIC.Text = oMailSubject.GenerateDigest(MailMessagePartTypeConstants.Message_All, EncodingMechanismTypeConstants.EncodeType_Base64);

            Cursor = Cursors.WaitCursor;

            //Put all things together
            if (oMailSubject.Prepare() != 1)
                MessageBox.Show("Failed to prepare mail subject");
                //These headers were moved after the Prepare method so that they don't get included in the encrypted message.

                // Put globally unique ID as Message-ID.
                oMailSubject.set_HeaderFieldValue("Message-ID", "");  //txtMessageID.Text
                // Put AS2 version.
                oMailSubject.set_HeaderFieldValue("AS2-Version", "1.0");
                // Put AS2-To header value.
                oMailSubject.set_HeaderFieldValue("AS2-To", "ToCompanyABC");
                // Put AS2-From header value.
                oMailSubject.set_HeaderFieldValue("AS2-From", "FromCompanyXYZ");

                // MDN can be requested to be sent by the following ways:
                //  - Synchronously - use the same connection to receive the MDN that was used to send the AS2 message. 
                //  - Asynchronously - use a different connection to receive the MDN.  

                if (!bSyncronous) // asyschronous
                    // For asynchronous MDN, must set a syntactically correct URI at 'Receipt-delivery-option'.
                    oMailSubject.set_HeaderFieldValue("Receipt-delivery-option", "");
                    // Request MDN Acknowledgment by adding "Disposition-Notification-To" header.
                    // Value must be present when requesting for MDN, but holds no meaning in an AS2 environment.
                    oMailSubject.set_HeaderFieldValue("Disposition-Notification-To", "<>");
                //This is the file that you would send by http or https
                oMailDoc.Save(sPath + @"\MailFolder\AS2EncryptedSignedMdnRequest.bin");

                TransmitFileByHttp(ref oMailDoc, "AS2EncryptedSignedMdnRequest.bin");

                if (bSyncronous) // MDN syschronous
                    mailMessage oMDN = oMailDoc.GetMDN();   // Gets the MDN that was received syncronously
                    oMDN.Save(sPath + @"MailFolder\Received_MDN.bin");  // Saves the MDN to a file
                    ReadMdnMessage(ref oMDN);
                else // MDN asyschronous
                    mailMessage oMDN = new mailMessage();
                    oMDN.Import(sPath + @"MailFolder\Received_MDN.bin");  //This is the MDN received asyncronously so it needs to be loaded into the oMDN object.
                    ReadMdnMessage(ref oMDN);
                } // if (bSyncronous)

            }  // if (oMailSubject.Prepare())


            Cursor = Cursors.Default;


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